In reverse chronological order I have been using Empower 2, Empower, Millennium (all those for both GC and HPLC), Water's 860 Vax based Expert Ease fro HPLC. I understand the structure, and being used to thinking in terms of relational databases find, it logical, easy to use, extremely powerful AND extremely flexible. You rarely have to deal with datafiles with user unfriendly names and paths.

The default ChemStation naming sequence is 001-0101. Chose to use the Agilent HPLC because of the ChemStation software so it stood idle. Agilent ChemStation revision B.04.01 includes usability and productivity enhancements as well as major improvements in compliance support. It has has been specially designed to match the advanced features of the Agilent 1200 Series Rapid Resolution LC system and the Agilent 7890 GC system.

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I can review and process data anyway way I want very simply. And everything is linked together conveniently but transparently. I only have to worry about high level stuff. I just got a new job where they don't like Water's and they just bought a new Agilent HPLC with Chemstation.

First doesn't Agilent know that the Multiple Document Interface (MDI) UI on Windows fell out of favor long ago???? That alone is causing me grief! Outside of actually doing an injection (I REALLY don't need cartoons for that.) I'm find this software amazing convoluted, unintuitive and limited in it's method part organization and data review capabilities! Separate on-line and off line apps???!!!

Maybe it's just my unfamiliarity with Chemstation. But in general i'm pretty good with software (both using and writing) and i'm ready to start pulling out my hair. So can I ask questions about it here? First question. Is it possible top overlay two chromatograms in review mode from different acquisition sequences?

In Empower i would just select any number of channels or results regardless of when they were acquired or processed and review them. If I want I can then easily look at some or all of the injection as overlays or stacked plots. I've figured out (more or less) how to do that for injections in a single acquired sequence in ChemStation.

But can I do it across sequences? Thanks, - Karen. Karen01 wrote: First question. Is it possible top overlay two chromatograms in review mode from different acquisition sequences? Under view, make sure you are in 'Full Menu. Then in Data Analysis, load the first data signal from its data subdirectory (in Sequence Parameters or Sample data name each day's data in a separate 1 Data subdirectory, such as 112310 for today's date).

Then select overlay (the next choice down from load signal), find the data directory where that data lies, and load that to overlay. Under Graphics, first selection, you can choose overlay or separated, and whether to zoom independently. You could also copy chromatogram xxx.D directories to a different data directory if that is easier. The default ChemStation naming sequence is 001-0101.D where the first three characters are the vial number, the first two after the dash are the LINE in the Sequence Table, and the last two are which injection on that line. Under view, make sure you are in 'Full Menu.